Difference between revisions of "MurF"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[cell wall synthesis]]}},
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{{SubtiWiki category|[[biosynthesis of cell wall components]]}},
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{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, W, X, Y)]]}},
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{{SubtiWiki category|[[essential genes]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[SigM regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[cell wall synthesis]]}},
 
{{SubtiWiki category|[[biosynthesis of cell wall components]]}},
 
{{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, W, X, Y)]]}},
 
{{SubtiWiki category|[[essential genes]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 16:55, 8 December 2010

  • Description: UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine synthetase

Gene name murF
Synonyms ydbQ
Essential yes PubMed
Product UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-
2,6- diaminopimeloyl-D-alanyl-D-alanine synthetase
Function peptidoglycan precursor biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Cell wall
MW, pI 49 kDa, 5.337
Gene length, protein length 1371 bp, 457 aa
Immediate neighbours ddl, cshA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MurF context.gif
This image was kindly provided by SubtiList










Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU04570

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine (according to Swiss-Prot)
  • Protein family: murCDEF family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure: 1GG4 (from Escherichia coli, 32% identity, 50% similarity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol Microbiol: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Jean van Heijenoort
Lipid intermediates in the biosynthesis of bacterial peptidoglycan.
Microbiol Mol Biol Rev: 2007, 71(4);620-35
[PubMed:18063720] [WorldCat.org] [DOI] (P p)