Mycoplasma

Methods

Cedric Blötz, Carole Lartigue, Yanina Valverde Timana, Estelle Ruiz, Bernhard Paetzold, Julia Busse, Jörg Stülke
Development of a replicating plasmid based on the native oriC in Mycoplasma pneumoniae.
Microbiology (Reading): 2018, 164(11);1372-1382
[PubMed:30252643] [WorldCat.org] [DOI] (I p)

Sebastian R Schmidl, Claudine Hames, Jörg Stülke
Expression of Mycoplasma proteins carrying an affinity tag in M. pneumoniae allows rapid purification and circumvents problems related to the aberrant genetic code.
Appl Environ Microbiol: 2007, 73(23);7799-801
[PubMed:17933931] [WorldCat.org] [DOI] (I p)

Sven Halbedel, Jörg Stülke
Tools for the genetic analysis of Mycoplasma.
Int J Med Microbiol: 2007, 297(1);37-44
[PubMed:17223385] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Julia Busse, Sebastian R Schmidl, Jörg Stülke
Regulatory protein phosphorylation in Mycoplasma pneumoniae. A PP2C-type phosphatase serves to dephosphorylate HPr(Ser-P).
J Biol Chem: 2006, 281(36);26253-9
[PubMed:16857667] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Jörg Stülke
Probing in vivo promoter activities in Mycoplasma pneumoniae: a system for generation of single-copy reporter constructs.
Appl Environ Microbiol: 2006, 72(2);1696-9
[PubMed:16461731] [WorldCat.org] [DOI] (P p)

Claudine Hames, Sven Halbedel, Oliver Schilling, Jörg Stülke
Multiple-mutation reaction: a method for simultaneous introduction of multiple mutations into the glpK gene of Mycoplasma pneumoniae.
Appl Environ Microbiol: 2005, 71(7);4097-100
[PubMed:16000825] [WorldCat.org] [DOI] (P p)

Metabolism

Cedric Blötz, Katrin Treffon, Volkhard Kaever, Frank Schwede, Elke Hammer, Jörg Stülke
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Front Microbiol: 2017, 8;1328
[PubMed:28751888] [WorldCat.org] [DOI] (P e)

Stephanie Großhennig, Till Ischebeck, Johannes Gibhardt, Julia Busse, Ivo Feussner, Jörg Stülke
Hydrogen sulfide is a novel potential virulence factor of Mycoplasma pneumoniae: characterization of the unusual cysteine desulfurase/desulfhydrase HapE.
Mol Microbiol: 2016, 100(1);42-54
[PubMed:26711628] [WorldCat.org] [DOI] (I p)

Liya Wang, Sebastian R Schmidl, Jörg Stülke
Mycoplasma pneumoniae thymidine phosphorylase.
Nucleosides Nucleotides Nucleic Acids: 2014, 33(4-6);296-304
[PubMed:24940683] [WorldCat.org] [DOI] (I p)

Stephanie Großhennig, Sebastian R Schmidl, G Schmeisky, Julia Busse, Jörg Stülke
Implication of glycerol and phospholipid transporters in Mycoplasma pneumoniae growth and virulence.
Infect Immun: 2013, 81(3);896-904
[PubMed:23297388] [WorldCat.org] [DOI] (I p)

Sebastian R Schmidl, Andreas Otto, Maria Lluch-Senar, Jaume Piñol, Julia Busse, Dörte Becher, Jörg Stülke
A trigger enzyme in Mycoplasma pneumoniae: impact of the glycerophosphodiesterase GlpQ on virulence and gene expression.
PLoS Pathog: 2011, 7(9);e1002263
[PubMed:21966272] [WorldCat.org] [DOI] (I p)

Pavel Dutow, Sebastian R Schmidl, Meike Ridderbusch, Jörg Stülke
Interactions between glycolytic enzymes of Mycoplasma pneumoniae.
J Mol Microbiol Biotechnol: 2010, 19(3);134-9
[PubMed:20924199] [WorldCat.org] [DOI] (I p)

Liya Wang, Claudine Hames, Sebastian R Schmidl, Jörg Stülke
Upregulation of thymidine kinase activity compensates for loss of thymidylate synthase activity in Mycoplasma pneumoniae.
Mol Microbiol: 2010, 77(6);1502-11
[PubMed:20860090] [WorldCat.org] [DOI] (I p)

Claudine Hames, Sven Halbedel, Michael Hoppert, Joachim Frey, Jörg Stülke
Glycerol metabolism is important for cytotoxicity of Mycoplasma pneumoniae.
J Bacteriol: 2009, 191(3);747-53
[PubMed:19028882] [WorldCat.org] [DOI] (I p)

Sven Halbedel, Hinnerk Eilers, Beate Jonas, Julia Busse, Michael Hecker, Susanne Engelmann, Jörg Stülke
Transcription in Mycoplasma pneumoniae: analysis of the promoters of the ackA and ldh genes.
J Mol Biol: 2007, 371(3);596-607
[PubMed:17586527] [WorldCat.org] [DOI] (P p)

The phosphotransferase system

Sven Halbedel, Julia Busse, Sebastian R Schmidl, Jörg Stülke
Regulatory protein phosphorylation in Mycoplasma pneumoniae. A PP2C-type phosphatase serves to dephosphorylate HPr(Ser-P).
J Biol Chem: 2006, 281(36);26253-9
[PubMed:16857667] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Jörg Stülke
Dual phosphorylation of Mycoplasma pneumoniae HPr by Enzyme I and HPr kinase suggests an extended phosphoryl group susceptibility of HPr.
FEMS Microbiol Lett: 2005, 247(2);193-8
[PubMed:15927419] [WorldCat.org] [DOI] (P p)

Sven Halbedel, Claudine Hames, Jörg Stülke
In vivo activity of enzymatic and regulatory components of the phosphoenolpyruvate:sugar phosphotransferase system in Mycoplasma pneumoniae.
J Bacteriol: 2004, 186(23);7936-43
[PubMed:15547265] [WorldCat.org] [DOI] (P p)

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur J Biochem: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)

Gregory S Allen, Katrin Steinhauer, Wolfgang Hillen, Jörg Stülke, Richard G Brennan
Crystal structure of HPr kinase/phosphatase from Mycoplasma pneumoniae.
J Mol Biol: 2003, 326(4);1203-17
[PubMed:12589763] [WorldCat.org] [DOI] (P p)

Katrin Steinhauer, Tanja Jepp, Wolfgang Hillen, Jörg Stülke
A novel mode of control of Mycoplasma pneumoniae HPr kinase/phosphatase activity reflects its parasitic lifestyle.
Microbiology (Reading): 2002, 148(Pt 10);3277-3284
[PubMed:12368461] [WorldCat.org] [DOI] (P p)

Katrin Steinhauer, Gregory S Allen, Wolfgang Hillen, Jörg Stülke, Richard G Brennan
Crystallization, preliminary X-ray analysis and biophysical characterization of HPr kinase/phosphatase of Mycoplasma pneumoniae.
Acta Crystallogr D Biol Crystallogr: 2002, 58(Pt 3);515-8
[PubMed:11856840] [WorldCat.org] [DOI] (P p)