NrgA

• Description: ammonium transporter, required at low ammonium concentration
  
  

Gene name	nrgA
Synonyms	amtB
Essential	no
Product	ammonium transporter
Function	ammonium uptake
MW, pI	42 kDa, 5.727
Gene length, protein length	1212 bp, 404 aa
Immediate neighbours	ywoB, nrgB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: NrgA-NrgB PubMed

• Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

• Structure:

• Swiss prot entry: Q07429

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: nrgA-nrgB

• Sigma factor: SigA

• Regulation: induced by ammonium limitation

• Regulatory mechanism: transcription activation by TnrA

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

References

1. Atkinson MR, Fisher SH (1991) Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J Bacteriol 173:23-27. PubMed
2. Wray, L. V., Atkinson, M. R. & Fisher, S. H. (1994). The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein. J Bacteriol 176, 108-114. PubMed
3. Detsch, C. & Stülke, J. (2003) Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB. Microbiology 149: 3289-3297. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed