NrgA

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  • Description: ammonium transporter, required at low ammonium concentration

Gene name nrgA
Synonyms amtB
Essential no
Product ammonium transporter
Function ammonium uptake
MW, pI 42 kDa, 5.727
Gene length, protein length 1212 bp, 404 aa
Immediate neighbours ywoB, nrgB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NrgA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot), membrane

Database entries

  • Structure:
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by ammonium limitation
  • Regulatory mechanism: transcription activation by TnrA
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks

References

  1. Atkinson MR, Fisher SH (1991) Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis. J Bacteriol 173:23-27. PubMed
  2. Wray, L. V., Atkinson, M. R. & Fisher, S. H. (1994). The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein. J Bacteriol 176, 108-114. PubMed
  3. Detsch, C. & Stülke, J. (2003) Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB. Microbiology 149: 3289-3297. PubMed
  4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed