PGP1331

From SubtiWiki
Revision as of 13:06, 16 March 2010 by MLehnik (talk | contribs) (Created page with ' '''general comments''' Integrative Plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor E.col…')
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search


general comments

Integrative Plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor E.coli: multicopy plasmid, select for ampicillin B. subtilis: no ori, select for spectinomycin

Hints for the usage amplify the last 600bp of your gene of interest, skip the Stopp-Codon of the gene in the revers primer take care that the integration is in-frame

Antibody dilute the anitbody 1:10000 (you can freeze the Blotto solution and reuse it)