Difference between revisions of "PGP382"

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[[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']]
 
[[File:PGP382_cloning_region.jpeg|250px|thumb|right|'''pGP382 cloning region: click to enlarge''']]
  
Vector for the overexpression of C-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
+
Vector for the overexpression of C-terminally Strep-tagged proteins in ''B. subtilis'', used for the [[SPINE]] technology
  
 
constructed in [[Stülke]] lab
 
constructed in [[Stülke]] lab

Revision as of 13:41, 8 May 2009

pGP382: click to enlarge
pGP382 cloning region: click to enlarge

Vector for the overexpression of C-terminally Strep-tagged proteins in B. subtilis, used for the SPINE technology

constructed in Stülke lab

in E. coli: ampicillin resistance

in B. subtilis: erythromycin resistance

based on pBQ200

similar vector: pGP380

sequencing primer:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘


Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. PubMed