Difference between revisions of "PolY1"

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= Categories containing this gene/protein =
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{{SubtiWiki category|[[DNA repair/ recombination]]}}
 
=The protein=
 
=The protein=
  

Revision as of 18:53, 30 November 2010

  • Description: translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis

Gene name polY1
Synonyms yqjH
Essential no
Product translesion synthesis (TLS-) DNA polymerase Y1
Function generation of mutations in stationary phase
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 46 kDa, 9.953
Gene length, protein length 1242 bp, 414 aa
Immediate neighbours gndA, mifM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YqjH context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU23870

Phenotypes of a mutant

reduced UV-induced mutagenesis PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

DNA repair/ recombination

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (homogeneous)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: constitutively expressed PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1111 (spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Andrea M Rivas-Castillo, Ronald E Yasbin, E Robleto, Wayne L Nicholson, Mario Pedraza-Reyes
Role of the Y-family DNA polymerases YqjH and YqjW in protecting sporulating Bacillus subtilis cells from DNA damage.
Curr Microbiol: 2010, 60(4);263-7
[PubMed:19924481] [WorldCat.org] [DOI] (I p)

Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
DNA polymerase I acts in translesion synthesis mediated by the Y-polymerases in Bacillus subtilis.
Mol Microbiol: 2005, 57(3);678-90
[PubMed:16045613] [WorldCat.org] [DOI] (P p)

Stéphane Duigou, S Dusko Ehrlich, Philippe Noirot, Marie-Françoise Noirot-Gros
Distinctive genetic features exhibited by the Y-family DNA polymerases in Bacillus subtilis.
Mol Microbiol: 2004, 54(2);439-51
[PubMed:15469515] [WorldCat.org] [DOI] (P p)

Huang-Mo Sung, Gabriel Yeamans, Christian A Ross, Ronald E Yasbin
Roles of YqjH and YqjW, homologs of the Escherichia coli UmuC/DinB or Y superfamily of DNA polymerases, in stationary-phase mutagenesis and UV-induced mutagenesis of Bacillus subtilis.
J Bacteriol: 2003, 185(7);2153-60
[PubMed:12644484] [WorldCat.org] [DOI] (P p)