PolY1

• Description: translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis
  
  

Gene name	polY1
Synonyms	yqjH
Essential	no
Product	translesion synthesis (TLS-) DNA polymerase Y1
Function	generation of mutations in stationary phase
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	46 kDa, 9.953
Gene length, protein length	1242 bp, 414 aa
Immediate neighbours	gndA, mifM
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU23870

Phenotypes of a mutant

reduced UV-induced mutagenesis PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

• Protein family: DNA polymerase type-Y family (according to Swiss-Prot)

• Paralogous protein(s): PolY2, UvrX, YozK-YobH

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • PolY1-DnaN (required for untargeted mutagenesis) PubMed
  • PolA-PolY1-DnaN PubMed

• Localization: cytoplasm (homogeneous)

Database entries

• Structure:

• UniProt: P54545

• KEGG entry: [3]

• E.C. number: 2.7.7.7

Expression and regulation

• Operon: polY1-gndA (according to DBTBS)

• Sigma factor:

• Regulation: constitutively expressed PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP1111 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: