Difference between revisions of "PrkD"

From SubtiWiki
Jump to: navigation, search
Line 15: Line 15:
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02030 prkD]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU02030 prkD]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=PrkD PrkD]
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 6.359   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 6.359   
Line 35: Line 37:
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
 
 
 
<br/><br/>
 
<br/><br/>
  
Line 64: Line 62:
  
 
=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
Line 84: Line 79:
 
* '''Kinetic information:'''
 
* '''Kinetic information:'''
  
* '''Domains:'''  
+
* '''[[Domains]]:'''  
  
 
* '''Modification:''' autophosphorylated on Thr-174 (''in vitro'')  {{PubMed|20389117}}
 
* '''Modification:''' autophosphorylated on Thr-174 (''in vitro'')  {{PubMed|20389117}}
  
* '''Cofactor(s):'''
+
* '''[[Cofactors]]:'''
  
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
 
* '''[[SubtInteract|Interactions]]:'''
 
* '''[[SubtInteract|Interactions]]:'''
 +
** [[AbrB]]-[[PrkD]] {{PubMed|24731262}}
  
 
* '''[[Localization]]:'''
 
* '''[[Localization]]:'''
Line 115: Line 111:
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 prkD] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ybdM_223219_223989_-1 prkD] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
Line 145: Line 141:
  
 
=References=
 
=References=
<pubmed> 20389117  12406230 21304896</pubmed>
+
<pubmed> 20389117  12406230 21304896 24731262</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:33, 16 April 2014

Gene name prkD
Synonyms ybdM
Essential no
Product protein kinase D
Function unknown
Gene expression levels in SubtiExpress: prkD
Interactions involving this protein in SubtInteract: PrkD
MW, pI 29 kDa, 6.359
Gene length, protein length 768 bp, 256 aa
Immediate neighbours ybzI, ybdN
Sequences Protein DNA DNA_with_flanks
Genetic context
YbdM context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrkD expression.png















Categories containing this gene/protein

protein modification, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU02030

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
    • phosphorylates DegS on Ser-76 (in vitro) PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification: autophosphorylated on Thr-174 (in vitro) PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP578 (aphA3), available in the Stülke lab
  • Expression vector:
    • pGP390 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab
    • for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP821, available in Stülke lab
    • for expression, purification in E. coli with N-terminal His-tag, in pWH844: pGP1407, available in Stülke lab
  • lacZ fusion: pGP830 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, Samira Zouhir, Rosa Grenha, Sylvie Nessler, Phillipe Noirot, Ivan Mijakovic
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties.
Mol Microbiol: 2014, 92(5);1129-41
[PubMed:24731262] [WorldCat.org] [DOI] (I p)

Carsten Jers, Ahasanul Kobir, Elsebeth Oline Søndergaard, Peter Ruhdal Jensen, Ivan Mijakovic
Bacillus subtilis two-component system sensory kinase DegS is regulated by serine phosphorylation in its input domain.
PLoS One: 2011, 6(2);e14653
[PubMed:21304896] [WorldCat.org] [DOI] (I e)

Nico Pietack, Dörte Becher, Sebastian R Schmidl, Milton H Saier, Michael Hecker, Fabian M Commichau, Jörg Stülke
In vitro phosphorylation of key metabolic enzymes from Bacillus subtilis: PrkC phosphorylates enzymes from different branches of basic metabolism.
J Mol Microbiol Biotechnol: 2010, 18(3);129-40
[PubMed:20389117] [WorldCat.org] [DOI] (I p)

Edwige Madec, Agnieszka Laszkiewicz, Adam Iwanicki, Michal Obuchowski, Simone Séror
Characterization of a membrane-linked Ser/Thr protein kinase in Bacillus subtilis, implicated in developmental processes.
Mol Microbiol: 2002, 46(2);571-86
[PubMed:12406230] [WorldCat.org] [DOI] (P p)