Difference between revisions of "RplT"

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(Expression and regulation)
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** binding of [[RplT]] to a RNA site in the ''[[infC]]'' leader region causes transcription termination {{PubMed|17289755}}  
+
** binding of [[RplT]] to a [[RNA switch]] in the ''[[infC]]'' leader region causes transcription termination {{PubMed|17289755}}  
  
* '''Additional information:'''  
+
* '''Additional information:'''
  
 
=Biological materials =
 
=Biological materials =

Revision as of 16:24, 1 May 2011

Gene name rplT
Synonyms
Essential yes PubMed
Product ribosomal protein L20
Function translation
MW, pI 13 kDa, 11.901
Gene length, protein length 357 bp, 119 aa
Immediate neighbours ysdA, rpmI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RplT context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

translation, RNA binding regulators, essential genes, membrane proteins

This gene is a member of the following regulons

RplT regulon, stringent response

The RplT regulon: infC-rpmI-rplT-ysdA

The gene

Basic information

  • Locus tag: BSU28850

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer
Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.
Nucleic Acids Res: 2007, 35(5);1578-88
[PubMed:17289755] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)