Difference between revisions of "RsmG"

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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
{{SubtiWiki regulon|[[ComK regulon]]}}
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{{SubtiWiki regulon|[[ComK regulon]]}},
 +
{{SubtiWiki regulon|[[DnaA regulon]]}}
  
 
=The gene=
 
=The gene=
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=== Additional information===
 
=== Additional information===
 
 
 
  
 
=The protein=
 
=The protein=
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsmG_4208870_4209589_-1 rsmG] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rsmG_4208870_4209589_-1 rsmG] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[ComK]]: transcription activation {{PubMed|11948146}}
 
** [[ComK]]: transcription activation {{PubMed|11948146}}
 +
** [[DnaA]]: transcription repression {{PubMed|26020636}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
<pubmed>17573471 11948146 11807071</pubmed>
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<pubmed>17573471 11948146 11807071 26020636</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 13:03, 1 June 2015

  • Description: 7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein

Gene name gidB
Synonyms
Essential no
Product 7-methylguanosine methyltransferase specific for the 16S rRNA
Function rRNA modification
Gene expression levels in SubtiExpress: rsmG
MW, pI 26 kDa, 6.603
Gene length, protein length 717 bp, 239 aa
Immediate neighbours noc, gidA
Sequences Protein DNA DNA_with_flanks
Genetic context
GidB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RsmG expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

ComK regulon, DnaA regulon

The gene

Basic information

  • Locus tag: BSU41000

Phenotypes of a mutant

low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: methylates the N7 position of nucleotide G535 in 16S rRNA PubMed
  • Protein family: RNA methyltransferase rsmG family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 156 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Janet L Smith, Alan D Grossman
In Vitro Whole Genome DNA Binding Analysis of the Bacterial Replication Initiator and Transcription Factor DnaA.
PLoS Genet: 2015, 11(5);e1005258
[PubMed:26020636] [WorldCat.org] [DOI] (I e)

Kenji Nishimura, Shanna K Johansen, Takashi Inaoka, Takeshi Hosaka, Shinji Tokuyama, Yasutaka Tahara, Susumu Okamoto, Fujio Kawamura, Stephen Douthwaite, Kozo Ochi
Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants.
J Bacteriol: 2007, 189(16);6068-73
[PubMed:17573471] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Jörg Sievers, Brian Raether, Marta Perego, Jeff Errington
Characterization of the parB-like yyaA gene of Bacillus subtilis.
J Bacteriol: 2002, 184(4);1102-11
[PubMed:11807071] [WorldCat.org] [DOI] (P p)