SigL

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Gene name sigL
Synonyms
Essential no
Product RNA polymerase sigma-54 factor (sigma-L)
Function utilization of arginin, acetoin and fructose,
required for cold adaptation
Gene expression levels in SubtiExpress: sigL
Interactions involving this protein in SubtInteract: SigL
MW, pI 49,5 kDa, 7.79
Gene length, protein length 1308 bp, 436 amino acids
Immediate neighbours yvfH, yvfG
Sequences Protein DNA DNA_with_flanks
Genetic context
SigL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SigL expression.png
















Categories containing this gene/protein

transcription, sigma factors and their control

This gene is a member of the following regulons

CcpA regulon

The SigL regulon

The gene

Basic information

  • Locus tag: BSU34200

Phenotypes of a mutant

The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
  • Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
  • Paralogous protein(s):

Transcription factors activating transcription at SigL-dependent promoters

AcoR, BkdR, LevR, RocR, YplP

Extended information on the protein

  • Kinetic information:
  • Domains:
    • DNA binding domain (H-T-H motif) (324–343)
    • pron box domain (413–421)
    • 3 x Compositional bias domain (6–21),(32–53),(112–136)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Transcription initiation by SigL-containing RNA polymerase requires the activity of ATP-hydrolyzing transcription activators.

Transcription factors activating transcription at SigL-dependent promoters

Expression and regulation

  • Operon: sigL (according to DBTBS)
  • Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

Reviews

Nan Zhang, Martin Buck
A perspective on the enhancer dependent bacterial RNA polymerase.
Biomolecules: 2015, 5(2);1012-9
[PubMed:26010401] [WorldCat.org] [DOI] (I e)

Victoria Shingler
Signal sensory systems that impact σ⁵⁴ -dependent transcription.
FEMS Microbiol Rev: 2011, 35(3);425-40
[PubMed:21054445] [WorldCat.org] [DOI] (I p)

M Buck, M T Gallegos, D J Studholme, Y Guo, J D Gralla
The bacterial enhancer-dependent sigma(54) (sigma(N)) transcription factor.
J Bacteriol: 2000, 182(15);4129-36
[PubMed:10894718] [WorldCat.org] [DOI] (P p)

M J Merrick
In a class of its own--the RNA polymerase sigma factor sigma 54 (sigma N).
Mol Microbiol: 1993, 10(5);903-9
[PubMed:7934866] [WorldCat.org] [DOI] (P p)


Original publications

Yun Yang, Vidya C Darbari, Nan Zhang, Duo Lu, Robert Glyde, Yi-Ping Wang, Jared T Winkelman, Richard L Gourse, Katsuhiko S Murakami, Martin Buck, Xiaodong Zhang
TRANSCRIPTION. Structures of the RNA polymerase-σ54 reveal new and conserved regulatory strategies.
Science: 2015, 349(6250);882-5
[PubMed:26293966] [WorldCat.org] [DOI] (I p)

Amit Sharma, Robert N Leach, Christopher Gell, Nan Zhang, Patricia C Burrows, Dale A Shepherd, Sivaramesh Wigneshweraraj, David Alastair Smith, Xiaodong Zhang, Martin Buck, Peter G Stockley, Roman Tuma
Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies.
Nucleic Acids Res: 2014, 42(8);5177-90
[PubMed:24553251] [WorldCat.org] [DOI] (I p)

Bogumiła C Marciniak, Monika Pabijaniak, Anne de Jong, Robert Dűhring, Gerald Seidel, Wolfgang Hillen, Oscar P Kuipers
High- and low-affinity cre boxes for CcpA binding in Bacillus subtilis revealed by genome-wide analysis.
BMC Genomics: 2012, 13;401
[PubMed:22900538] [WorldCat.org] [DOI] (I e)

Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774] [WorldCat.org] [DOI] (P p)

Soo-Keun Choi, Milton H Saier
Regulation of sigL expression by the catabolite control protein CcpA involves a roadblock mechanism in Bacillus subtilis: potential connection between carbon and nitrogen metabolism.
J Bacteriol: 2005, 187(19);6856-61
[PubMed:16166551] [WorldCat.org] [DOI] (P p)

N O Ali, J Bignon, G Rapoport, M Debarbouille
Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.
J Bacteriol: 2001, 183(8);2497-504
[PubMed:11274109] [WorldCat.org] [DOI] (P p)

M Débarbouillé, I Martin-Verstraete, F Kunst, G Rapoport
The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria.
Proc Natl Acad Sci U S A: 1991, 88(20);9092-6
[PubMed:1924373] [WorldCat.org] [DOI] (P p)