Difference between revisions of "Sr1"

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(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
+
* '''Mutant:''' 168 sr1::cat, available in [[Brantl]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
 
* '''GFP fusion:'''
 
 
* '''Antibody:'''
 
 
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=

Revision as of 20:10, 14 January 2009

  • Description: small regulatory RNA controlling AhrC expression

Gene name sr1
Synonyms
Essential no
Product small regulatory RNA
Function control of arginine metabolism
pI, MW not applicable
Gene length, protein length 205 bp, not applicable
Immediate neighbours slp, speA
Gene sequence (+200bp)
Genetic context
File:GenE context.gif




The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry:

Additional information

Located between the slp and speA genes.


The RNA

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: hybridizes to ahrC mRNA and inhibits AhrC translation. PubMed

Extended information on the RNA

  • Kinetic information
  • Localization

Database entries

  • Structure:

Additional information

Information on the structure of the RNA can be found in Heidrich et al., 2006

Expression and regulation

  • Regulation: Repressed in the presence of glucose by CcpN PubMed
  • Regulatory mechanism: transcription repression PubMed
  • Additional information:

Biological materials

  • Mutant: 168 sr1::cat, available in Brantl lab
  • Expression vector:
  • lacZ fusion:

Labs working on this gene/protein

Sabine Brantl, Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany homepage

Your additional remarks

References

  1. Licht et al. (2005) Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in Bacillus subtilis. Mol. Microbiol. 58: 189-206. PubMed
  2. Heidrich et al. (2006) The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism. Mol. Microbiol. 62: 520-536. PubMed
  3. Heidrich et al. (2007) In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA. Nucl. Acids Res. 35: 4331-4346. PubMed