YabT

• Description: Ser/Thr kinase, controls DNA integrity during spore development
  
  

Gene name	yabT
Synonyms
Essential	no
Product	Ser/Thr kinase
Function	control of DNA integrity during spore development
Gene expression levels in SubtiExpress: yabT
Interactions involving this protein in SubtInteract: YabT
MW, pI	37 kDa, 9.943
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yabS, tilS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, sporulation proteins

This gene is a member of the following regulons

SigF regulon

The gene

Basic information

• Locus tag: BSU00660

Phenotypes of a mutant

• increased sensitivity to DNA damage during spore development PubMed

Database entries

• BsubCyc: BSU00660

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • ATP + a protein = ADP + a phosphoprotein (according to Swiss-Prot)
  • phosphorylation of RecA on Ser-2 PubMed

• Protein family: protein kinase domain (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:
  • autophosphorylation is stimulated by non-specific binding to DNA PubMed

• Interactions:
  • YabT-RecA PubMed
  • AbrB-YabT PubMed

• Localization:
  • colocalizes strongly with the septal membrane separating the mother cells from the forespore PubMed

Database entries

• BsubCyc: BSU00660

• Structure:

• UniProt: P37562

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yabT PubMed

• Sigma factor: SigF PubMed

• Regulation: expressed early during sporulation in the forespore (SigF) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP577 (erm), available in Jörg Stülke's lab

• Expression vector:
  • purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP391, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal Strep-tag, in pGP172: pGP823, available in Jörg Stülke's lab
  • purification from E. coli with N-terminal His-tag, in pWH844: pGP1408, available in Jörg Stülke's lab

• lacZ fusion:
• translational lacZ fusion (in pAC7): pGP831, available in Jörg Stülke's lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lei Shi, Nathalie Pigeonneau, Vaishnavi Ravikumar, Paula Dobrinic, Boris Macek, Damjan Franjevic, Marie-Francoise Noirot-Gros, Ivan Mijakovic
Cross-phosphorylation of bacterial serine/threonine and tyrosine protein kinases on key regulatory residues.
Front Microbiol: 2014, 5;495
[PubMed:25278935] [WorldCat.org] [DOI] (P e)

Ahasanul Kobir, Sandrine Poncet, Vladimir Bidnenko, Olivier Delumeau, Carsten Jers, Samira Zouhir, Rosa Grenha, Sylvie Nessler, Phillipe Noirot, Ivan Mijakovic
Phosphorylation of Bacillus subtilis gene regulator AbrB modulates its DNA-binding properties.
Mol Microbiol: 2014, 92(5);1129-41
[PubMed:24731262] [WorldCat.org] [DOI] (I p)

Vladimir Bidnenko, Lei Shi, Ahasanul Kobir, Magali Ventroux, Nathalie Pigeonneau, Céline Henry, Alain Trubuil, Marie-Françoise Noirot-Gros, Ivan Mijakovic
Bacillus subtilis serine/threonine protein kinase YabT is involved in spore development via phosphorylation of a bacterial recombinase.
Mol Microbiol: 2013, 88(5);921-35
[PubMed:23634894] [WorldCat.org] [DOI] (I p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)