Difference between revisions of "YloU"

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(Biological materials)
(Biological materials)
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** pGP1324 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
** pGP1324 (N-terminal His-tag, in [[pWH844]]), available in [[Jörg Stülke]]'s lab
  
       
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
  

Revision as of 09:15, 19 September 2013

  • Description: similar to alkaline-shock protein

Gene name yloU
Synonyms
Essential yes PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yloU
MW, pI 13 kDa, 7.077
Gene length, protein length 360 bp, 120 aa
Immediate neighbours rpmB, yloV
Sequences Protein DNA DNA_with_flanks
Genetic context
YloU context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YloU expression.png















Categories containing this gene/protein

proteins of unknown function, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15830

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asp23 family (according to Swiss-Prot)
  • Paralogous protein(s): YqhY

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
    • GP1476 (chromosomal yloU-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
    • pGP1323 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
    • pGP1324 (N-terminal His-tag, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Fabian M Commichau, Nico Pietack, Jörg Stülke
Essential genes in Bacillus subtilis: a re-evaluation after ten years.
Mol Biosyst: 2013, 9(6);1068-75
[PubMed:23420519] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Helena B Thomaides, Ella J Davison, Lisa Burston, Hazel Johnson, David R Brown, Alison C Hunt, Jeffery Errington, Lloyd Czaplewski
Essential bacterial functions encoded by gene pairs.
J Bacteriol: 2007, 189(2);591-602
[PubMed:17114254] [WorldCat.org] [DOI] (P p)