citR

168

transcriptional repressor of [gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]

locus: BSU_09430

Molecular weight: 35.44 kDa

pI: 8.67

Protein length: 291 aa

Gene length: 876 bp

function: regulation of the minor citrate synthase

product: transcriptional repressor ([wiki|LysR family])

essential: no

synonyms: citR

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 1,020,073 → 1,020,948

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The protein

Protein family

• [wiki|LysR family] (according to UniProt)

[wiki|Domains]

• [wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)

Structure

• [AF|P39127]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]

  description: [Pubmed|8045898]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8045899], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP1283 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]'')::''aphA3'', available in [wiki|Jörg Stülke]'s lab
• GP1753 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]-[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA]'')::''aphA3'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
• GP2360 Δ(''[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]-[gene|6684F6083B001E9FDC5967799B715AE966B83E1D|citA])''::''erm'', the resistance cassette can be cut out by introducing the ''cre''-rekombinase into the chromosom of ''B. subtilis'', available in [wiki|Jörg Stülke]'s lab
• BKE09430 (Δ[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA,  downstream forward: _UP4_TAGGAGCAACCAAAACGCCT
• BKK09430 (Δ[gene|E7E0B170D1EEECE4E4EDC29B0399CB48253E9A17|citR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09430 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTATTCTCCCTCTGA,  downstream forward: _UP4_TAGGAGCAACCAAAACGCCT

Expression vectors

• pGP2264 (N-terminal His-tag, purification from ''E. coli'', in [wiki|pETM-11]), available in [wiki|Jörg Stülke]'s lab
• pGP1023 (C-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP574]), available in [wiki|Jörg Stülke]'s lab
• pGP1029 (N-terminal Strep-tag, purification from ''B. subtilis'', in [wiki|pGP380]), available in [wiki|Jörg Stülke]'s lab
• pGP1030 (C-terminal Strep-tag, purification from ''B. subtilis'', in [wiki|pGP382]), available in [wiki|Jörg Stülke]'s lab
• pGP2260 (N-terminal Strep-tag, purification from ''E. coli'', in [wiki|pGP172]), available in [wiki|Jörg Stülke]'s lab
• pGP2262 (integration into [gene|DBBA549C2D71CF628E92AC7B659EA276E227D742|ganA], expression under the control of the xylose-inducible PxylA promoter in ''B. subtilis'', in [wiki|pGP888]), available in [wiki|Jörg Stülke]'s lab

References

• 8045898,8045899