fnr

168

transcriptional regulator of anaerobic genes

locus: BSU_37310

Molecular weight: 27.07 kDa

pI: 5.99

Protein length: 238 aa

Gene length: 717 bp

function: regulation of anaerobiosis, fermentation and overflow metabolism

product: transcription regulator (Crp family)

essential: no

synonyms: fnr

List of homologs in different organisms, belongs to COG0664 (Galperin et al., 2021)

Gene

Coordinates: 3,831,512  3,832,228

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The protein

[wiki|Domains]

• HTH crp-type domain (aa 145-219) (according to UniProt)

[wiki|Cofactors]

• [4Fe-4S] cluster [pubmed|29292548]

Structure

• [AF|P46908]

Effectors of protein activity

• contains an iron-sulfur cluster

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]

  description: [Pubmed|8846791]

  regulation

  • ''[protein|search|fnr]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

  regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]: activation, [Pubmed|10972836], in [regulon|protein:1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD regulon]
  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|F14481DAA196868ACC1DE9EC8CBE593A2CBB6BE4|narK]-[gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]

  description: [Pubmed|8846791]

  regulation

  • ''[protein|search|fnr]'': expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|10972836]

  regulatory mechanism

  • [protein|EC6697D5D945B7E5083AFED9218748763C443278|nsrR]: repression, [Pubmed|16885456], in [regulon|protein:EC6697D5D945B7E5083AFED9218748763C443278|nsrR regulon]
  • [protein|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]: activation, [Pubmed|8846791,16428414], in [regulon|protein:7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8846791], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE37310 ([gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA,  downstream forward: _UP4_TGAAAAAAACAAGGACAGCG
• BKK37310 ([gene|7165CC59CDAF64AAE2D591936860304A53BE5DF6|fnr]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAGCTCACCTCGAAGAA,  downstream forward: _UP4_TGAAAAAAACAAGGACAGCG

References

Reviews

• 23046954,30225854

Other original publications

• 8682783,10972836,16885456,8846791,21068385,30225854

The [wiki|Fnr regulon]

• 16428414