glnR

glnR
168

transcriptional repressor ([wiki|MerR family]) of the [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] operon

locus
BSU_17450
Molecular weight
15.69 kDa
pI
9.73
Protein length
Gene length
function
regulation of glutamine synthesis
product
transcription repressor ([wiki|MerR family])
essential
no
synonyms
glnR

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0789 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,877,959  1,878,366
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The protein
Protein family
[wiki|MerR family]
[wiki|Domains]
[wiki|HTH merR-type domain] (aa 11-79) (according to UniProt)
[wiki|Cofactors]
feedback-inhibited [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18195355]
Structure
[PDB|4R4E] (the GlnR-DNA complex) [Pubmed|25691471]
[PDB|7TFC] (the [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]-[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] (C tail peptide) complex) [pubmed|35778410]
[AF|P37582]
Effectors of protein activity
activity is enhanced upon interaction of the C-terminal domain with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] [Pubmed|18331450]
Expression and Regulation
Operons
genes
[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]
description
[Pubmed|8636055]
regulation
expressed in the absence of glutamine ([protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]) [Pubmed|8636055]
the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, in complex with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2906311], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]→[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]

2024-02-21 23:41:02

Jstuelk

48

071583dc6a41a10d9b12ab56cf295c4d4fbfe350

168F5BFAABEE27136E7759E8FF655AA525A270D1

genes
[gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]-[gene|14EFA3BF4E5DD5368EF02B0733300CEF7A4E0DC7|ynbB]-[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]
description
[Pubmed|22383849]
regulation
the mRNA is processed between [gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR] and [gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|ricA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|ricF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|ricT] complex [Pubmed|29794222]
Open in new tab

[gene|9F6B78C1932FB56F7F71430DB16BC862A312407B|ynbA]→[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]

2024-01-20 23:07:57

Jstuelk

77

d45811f6bc7bef80a041d78aaa209f36dccdee6a

2408FECBCAE7255480B230470AF58DDA9F084F5A

genes
[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]
description
[Pubmed|22383849]
regulatory mechanism
[protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA]: repression, [Pubmed|8799114], in [regulon|protein:857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|tnrA regulon]
[protein|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]: repression, in complex with [protein|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA], in [regulon|protein:641C4BDD9702804642E1753A9C779E80FABB3919|glnR regulon]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2906311], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
Open in new tab

[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]

2024-03-03 06:42:00

Jstuelk

37

5d85452d00a4a5ee1c424fb007732e6f9725ac29

8EFC739FE47C5DE47CAAF68DE72A5F61BAB001FA

additional information
the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]
Biological materials
Mutant
BP148 (del(''[gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]-[gene|68F4E792B99F86AE9E8F06D2200E128937331F5D|glnA]'')::''cat''), available in [wiki|Fabian Commichau]'s lab
BKE17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT,  downstream forward: _UP4_TAATCCGTTAGCAACTGTTT
BKK17450 ([gene|641C4BDD9702804642E1753A9C779E80FABB3919|glnR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17450 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTCAATTTCCTCCTTTT,  downstream forward: _UP4_TAATCCGTTAGCAACTGTTT
lacZ fusion
pGP189 (in [wiki|pAC7]), available in [wiki|Jörg Stülke]'s lab
labs
[wiki|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]
References
Reviews
22625175
Original Publications
12374841,9287005,8799114,18195355,2573733,8636055,19233925,2906311,18331450,16547045,23535029,25691471,25755103,26883633,28835263,29794222,35778410

641C4BDD9702804642E1753A9C779E80FABB3919

Page visits: 7479

Time of last update: 2024-03-28 20:17:47

Author of last update: Jstuelk