kre

168

[protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] repressor, stimulates degradation of the [gene|search|comK ]mRNA

locus: BSU_14020

Molecular weight: 17.75 kDa

pI: 10.17

Protein length: 154 aa

Gene length: 465 bp

function: control of [protein|search|ComK ]accumulation and of bistable competence gene expression

product: [protein|search|ComK ]repressor

essential: no

synonyms: kre, ykyB

List of homologs in different organisms

Gene

Coordinates: 1,474,560  1,475,024

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The protein

Catalyzed reaction/ biological activity

• stimulates [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK] mRNA degradation [Pubmed|26110430]
• controls stability of several mRNAs (see [wiki|Kre regulon]) [Pubmed|26110430]

Structure

• [AF|P42430]

[wiki|Localization]

• cytoplasm [Pubmed|26110430]

Expression and Regulation

Operons

• genes: [gene|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]

  description: [Pubmed|8169223]

  regulation

  • repressed in competent cells ([wiki|ComK]) [Pubmed|26110430]

  regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]: repression, [Pubmed|26110430], in [regulon|protein:08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK regulon]

Biological materials

Mutant

• MGNA-A764 (ykyB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/764 NBRP B. subtilis, Japan]
• PG479 (ykyB::erm), available in [wiki|Leendert Hamoen]'s and [wiki|Jörg Stülke]'s labs [Pubmed|26110430]
• GP2533 ([gene|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]::''erm''), available in [wiki|Jörg Stülke]'s lab
• BKE14020 ([gene|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCTCTCCTTTGCT,  downstream forward: _UP4_TAAAATAAAAACAGCCGTGC
• BKK14020 ([gene|65E2F7197D46F5C884CC96018EE4F3EEE94FFA18|kre]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATTTCTCTCCTTTGCT,  downstream forward: _UP4_TAAAATAAAAACAGCCGTGC

References

Reviews

• 26110607

Original Publications

• 8169223,26110430