liaR

168

two-component response regulator, regulation of the [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR] operon in response to bacitracin

locus: BSU_33080

Molecular weight: 22.98 kDa

pI: 4.96

Protein length: 211 aa

Gene length: 636 bp

function: regulation of the [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR] operon

product: two-component response regulator

essential: no

synonyms: liaR, yvqC

List of homologs in different organisms, belongs to COG2197 (Galperin et al., 2021)

Gene

Coordinates: 3,394,422  3,395,057

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The protein

Catalyzed reaction/ biological activity

• regulation of the  ''[gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]'' operon in response to bacitracin

[wiki|Domains]

• [wiki|Response regulatory domain] (aa 3-119) (according to UniProt)
• [wiki|HTH luxR-type domain] (aa 143-208) (according to UniProt)

Structure

• [PDB|5HEV] ([protein|search|LiaR ]from Enterococcus faecium, 62% identity) [pubmed|27670715]
• [AF|O32197]

Modification

• phosphorylation on a Asp residue by [protein|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]

Paralogous protein(s)

• [protein|DD799ED3EB79457C27ED30C7B4DBBC2C8F962191|yhcZ], [protein|EFEAF09E4449A022A7323450FDED9458426A0080|ydfI], [protein|23F365C23BDEE02D42C9355FC7D2A65DAF9F79ED|yxjL], [protein|387EF370CE24F7A3C20789A57329A02EBED46F53|lnrK]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]

  description: [Pubmed|16816187]

  regulation

  • ''[protein|search|liaG]'': constitutive

  regulatory mechanism

  • [protein|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]: activation, [Pubmed|16816187], in [regulon|protein:49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|15273097], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

• genes: [gene|B6D1159454969D1D578E05D5CE2259E079688510|liaI]-[gene|AE4BF12C368468C553EED7A696D5EFC63F56CA39|liaH]-[gene|A71B0392FFF70EFFC096E7E9BD5FE30792821CDD|liaG]-[gene|C107A958DFA2B0DA4837E2F9F648CD329A3116AC|liaF]-[gene|9A1871BD853254EEA8B4E78CA187F9315CBDF43D|liaS]-[gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]

  description: [Pubmed|14651641]

  regulation

  • ''[protein|search|liaG]'': constitutive

Biological materials

Mutant

• MGNA-B034 (yvqC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1033 NBRP B. subtilis, Japan]
• BKE33080 ([gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE33080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCCCATTCTGACCATTTCAT,  downstream forward: _UP4_AATCATCTCGTGAATTAGGC
• BKK33080 ([gene|49E70A20CC05DBE485953C0AE34E634EFB33C3B2|liaR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK33080 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CCCCATTCTGACCATTTCAT,  downstream forward: _UP4_AATCATCTCGTGAATTAGGC

labs

• [wiki|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]

References

Reviews

• 27344142

Original Publications

• 18394148,19164152,15273097,17921301,10094672,19164157,14651641,17600057,17660417,16816187,15101989,17660417,20057163,20817675,20639339,23279150,22964256,22092710,23326432,27791134,27856233,27670715,29185696,32117169,30848888,32349670,33114184