spoVD

168

mother-cell specific class B penicillin-binding protein (spore cortex)

locus: BSU_15170

Molecular weight: 71.08 kDa

pI: 8.85

Protein length: 646 aa

Gene length: 1941 bp

function: synthesis of spore peptidoglycan

product: penicillin-binding protein (spore cortex)

essential: no

synonyms: spoVD

List of homologs in different organisms, belongs to COG0768 (Galperin et al., 2021)

Gene

Coordinates: 1,584,214  1,586,154

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The protein

Catalyzed reaction/ biological activity

• synthesis of spore peptidoglycan [pubmed|8289242]
• protects [protein|5DE602D97D903D9AE38ED05D5A5F1B3A2DC1D58E|spoVE] from proteolytic degradation [Pubmed|20417640]

Protein family

• [wiki|transpeptidase family] (according to UniProt)

[wiki|Domains]

• C-terminal [wiki|PASTA domain] (aa 580-638) [Pubmed|25481876]

Structure

• [PDB|1PYY] (PBP2B from Spreptococcus pneumoniae, 26% identity) [pubmed|12923202]
• [AF|Q03524]

Effectors of protein activity

• intramolecular disulfide bonds between two Cys residues are reduced by [protein|0B299F9459023306FA91298A6162A09E4A87C3B2|stoA], this is rquired for activity of SpoVD [Pubmed|19919673]

Paralogous protein(s)

• [protein|EBEFF9E0A524DCDA19382E5401B923B805E4C559|pbpB]

[wiki|Localization]

• intermembrane space that separates forespores from mother cells [Pubmed|19919673]
• outer forespore membrane [pubmed|20417640]

Expression and Regulation

Operons

• genes: [gene|38FD9805815BBDDD83789F0F402C0FB221B65FF4|spoVD]

  description: [Pubmed|8436954]

  regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,15758244]

  regulatory mechanism

  • [protein|90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID]: repression, [Pubmed|9006059], in [regulon|protein:90DCE4F286D2C151D8BDBF0E024E6BBEC94E2397|spoIIID regulon]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]: sigma factor, [Pubmed|15699190,15758244], in [regulon|protein:3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE regulon]

Biological materials

Mutant

• 1A975 ( ''spoVD''::''cat''), [Pubmed|19212404], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A975&Search=1A975 BGSC]
• BKE15170 ([gene|38FD9805815BBDDD83789F0F402C0FB221B65FF4|spoVD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE15170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT,  downstream forward: _UP4_TGATTCGGGCTGCCTATTCT
• BKK15170 ([gene|38FD9805815BBDDD83789F0F402C0FB221B65FF4|spoVD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK15170 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGAGACCGTTCACTCCTT,  downstream forward: _UP4_TGATTCGGGCTGCCTATTCT

References

Reviews

• 19919674,33709487,35638784,36411352

Original Publications

• 9006059,8289242,15699190,15758244,8436954,9006059,19919673,20417640,23789716,25481876,28383125,29661861,12923202,33573199