yhfT

168

similar to long-chain fatty-acid-CoA ligase, involved in surfactin production

locus: BSU_10360

Molecular weight: 52.71 kDa

pI: 5.95

Protein length: 479 aa

Gene length: 1440 bp

function: unknown

product: unknown

essential: no

synonyms: yhfT

List of homologs in different organisms, belongs to COG0318 (Galperin et al., 2021)

Gene

Coordinates: 1,110,479  1,111,918

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The protein

Protein family

• [wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Structure

• [PDB|5BUQ] ([protein|16412BAB04BBF9FD1C3653328923687874C81868|menE], 29% identitiy) [Pubmed|26276389]
• [AF|O07619]

Paralogous protein(s)

• [protein|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI], [protein|7AC5217CA35F8E134ABB35D74F1BA5E684D2E058|lcfA], [protein|41B879EF69E2D4F5C1896A4709567B88FD8AC5B7|lcfB]

Expression and Regulation

Operons

• genes: [gene|22215066A7BEA4E53189DE6639C19D1AD86DA7F4|panU]-[gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]

  description: [Pubmed|22383849]

  regulation

  • repressed in the presence of biotin ([protein|search|BirA]) [Pubmed|11717296]

  regulatory mechanism

  • [protein|F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA]: repression, [Pubmed|11717296], in [regulon|protein:F761DBCE3429C99159F8F7CD8596FD9CF2D0589D|birA regulon]

  additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]

• genes: [gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]-[gene|CF6B79564DBED3C6CAF64D01BD01D2A03675F16F|yhfS]-[gene|73CB11C654E7A92D6F59B00E0710D3AA1FD2F099|yhfR]

  description: [Pubmed|22383849]

  additional information

  • weakly expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/10869096 PubMed]

Biological materials

Mutant

• MGNA-B280 (yhfT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1279 NBRP B. subtilis, Japan]
• available in [wiki|Mohamed Marahiel]'s lab [Pubmed|20797616]
• BKE10360 ([gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT,  downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA
• BKK10360 ([gene|5050649605DD782AF10FC0AEEA5F14036EC0197D|yhfT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GGTATGAGTAATTGTCATGT,  downstream forward: _UP4_CTGGAAGAGAGTGTACAGTA

References

• 20797616,22383849,12368242