PHT255 - Revision history

Bzhu at 11:36, 28 August 2017

2017-08-28T11:36:06Z

Bzhu at 09:25, 28 August 2017

2017-08-28T09:25:36Z

Bzhu: Created page with "{{ContentDisclamer}} 600px 230px The pHT255 vector contains the coding sequence for a Strep tag and allows high-level intracellular p..."

2017-08-21T13:49:54Z

Created page with "{{ContentDisclamer}} 600px 230px The pHT255 vector contains the coding sequence for a Strep tag and allows high-level intracellular p..."

New page

{{ContentDisclamer}}

[[File:PHT255.PNG|600px]]
[[File:Legend.png|230px]]

The pHT255 vector contains the coding sequence for a Strep tag and allows high-level intracellular production of recombinant C-terminal Strep tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.
The expression is controlled by the strong P''grac100'' promoter, consisting of the ''groeESL'' promoter of ''B. subtilis'' with improved regulatory elements fused to the ''lac'' operator allowing their induction by IPTG. Nucleotides were optimized at the conserved regions of the ''groESL'' promoter including the UP element, the −35 and the −15 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein of ''B. subtilis'' (Phan ''et al''., 2011). pHT255 is an ''E. coli / B. subtilis'' shuttle vector, that provides ampicillin resistance to ''E.coli'' and chloramphenicol resistance to ''B. subtilis''.

=== Features ===

* Strong promoter with improved regulatory elements
* Enhanced amount of produced recombinant proteins
* IPTG inducible gene expression
* C-terminal Strep tag
* ''E. coli / B. subtilis'' shuttle vector

The pHT255 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]

== References ==

<pubmed>16125412, 22100269, 25990516</pubmed>

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 * ''E. coli / B. subtilis'' shuttle vector
-The pHT255 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)]
+The pHT255 vector is available from [https://www.mobitec.com/cms/products/vector_systems.html MoBiTec GmbH (Göttingen)][[File:Mobiteclogo.png|100px]]
 == References ==
 <pubmed>16125412, 22100269, 25990516</pubmed>

@@ Line 2: / Line 2: @@
 [[File:PHT255.PNG|600px]]
-[[File:Legend.png|230px]]
+[[File:PHT255-Legende.jpg|300px]]
 The pHT255 vector contains the coding sequence for a Strep tag and allows high-level intracellular production of recombinant C-terminal Strep tagged protein with ''B. subtilis''. The tag can be used for detection or purification of the produced protein of interest.