MutY

• Description: A/G-specific adenine glycosylase, error prevention oxidized guanine system, releases adenines from 8-oxo-G:A mismatches
  
  

Gene name	mutY
Synonyms	yfhQ
Essential	no
Product	A/G-specific adenine glycosylase
Function	DNA repair
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	41 kDa, 6.044
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	yfhP, yfhS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

YfhP regulon

The gene

Basic information

• Locus tag: BSU08630

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: releases adenines from 8-oxo-G:A mismatches

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1RRS (complex with DNA, Geobacillus stearothermophilus)

• UniProt: O31584

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mutY-fabL-sspE PubMed

• Sigma factor:
  • SigA PubMed
  • SigG PubMed

• Regulation:
  • expression is constitutive throughout growth PubMed
  • expression is fivefold increased in a mutS-mutL mutant PubMed
  • YfhP may act as a negative transcription regulator of the mutY-fabL-sspE operon during exponential growth PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Luz E Vidales, Lluvia C Cárdenas, Eduardo Robleto, Ronald E Yasbin, Mario Pedraza-Reyes
Defects in the error prevention oxidized guanine system potentiate stationary-phase mutagenesis in Bacillus subtilis.
J Bacteriol: 2009, 191(2);506-13
[PubMed:19011023] [WorldCat.org] [DOI] (I p)

Hiroki Yamamoto, Masao Mori, Junichi Sekiguchi
Transcription of genes near the sspE locus of the Bacillus subtilis genome.
Microbiology (Reading): 1999, 145 ( Pt 8);2171-2180
[PubMed:10463184] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed