csn

168

chitosanase

locus: BSU_26890

Molecular weight: 31.34 kDa

pI: 8.89

Protein length: 277 aa

Gene length: 834 bp

function: chitin degradation

product: chitosanase

essential: no

ec: 3.2.1.132

synonyms: csn

List of homologs in different organisms

Gene

Coordinates: 2,747,984 → 2,748,817

The protein

Catalyzed reaction/ biological activity

• Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan (according to UniProt)

Protein family

• glycosyl hydrolase 46 family (single member, according to UniProt)

Structure

• [PDB|7C6D] [pubmed|33744813]
• [AF|O07921]

[wiki|Localization]

• extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]

Expression and Regulation

Operons

• genes: [gene|EDE47BF726A4D2F0A4E1D9481C97576650339F84|csn]

  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [pubmed|20817675], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]

Biological materials

Mutant

• MGNA-A240 (csn::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/240 NBRP B. subtilis, Japan]
• BKE26890 (Δ[gene|EDE47BF726A4D2F0A4E1D9481C97576650339F84|csn]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE26890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCACTTCCCCTTTCTA,  downstream forward: _UP4_TAAGCAGTGAGGAAAAGAAA
• BKK26890 (Δ[gene|EDE47BF726A4D2F0A4E1D9481C97576650339F84|csn]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK26890 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCACTTCCCCTTTCTA,  downstream forward: _UP4_TAAGCAGTGAGGAAAAGAAA

References

• 11065371,18957862,22031025,20817675,12401130,33744813,37099965,37705423,38349745,40004057