recU

168

Holliday junction resolvase, DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] activities, required for efficient survival and replication restart after replication-transcription conflicts, required for rescuing the bacterial replisome at a nick

locus: BSU_22310

Molecular weight: 23.81 kDa

pI: 9.51

Protein length: 206 aa

Gene length: 621 bp

function: [wiki|DNA repair/ recombination] and [wiki|chromosome segregation]

product: Holliday junction resolvase

essential: no

synonyms: recU, prfA, yppB, jopB

List of homologs in different organisms, belongs to COG3331 (Galperin et al., 2021)

Gene

Coordinates: 2,340,802 → 2,341,422

Phenotypes of a mutant

• a [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] double mutant is not viable [pubmed|16020779]
• a [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ] double mutant is not viable [pubmed|16020779]
• the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|16020779,24770420]
• loss of plasmid transformation, this can be suppressed by deletion of [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] [pubmed|30050509]
• reduced survival after [category|SW.3.1.1|DNA replication] arrest imposed by inhibition of [protein|FB97DF0C147FB29944F60214CB9BC1803861DAA0|polC] activity [pubmed|36574412]

The protein

Catalyzed reaction/ biological activity

• binds Holliday junctions with high affinity [Pubmed|24770420]
• inhibits [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] filament growth, facilitates [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly from preformed filaments [pubmed|30050509]
• endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction) (according to UniProt)
• promotes a net [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] disassembly from viral ssDNAs not homologous to the host genome [pubmed|31876108]

Protein family

• RecU family (single member, according to UniProt)

Structure

• [PDB|1ZP7] [Pubmed|16154091]
• [AF|P39792]

Effectors of protein activity

• [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-mediated Holliday junction resolution is stimulated by [protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-ATP [Pubmed|24770420]
• [protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] [pubmed|34768753]

Paralogous protein(s)

• [protein|AD1F39823ABCE2A222259B879513752942C59590|mfd]

[wiki|Interactions]

• part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome [pubmed|24770420]

Expression and Regulation

Operons

• genes: [gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]

  description: [Pubmed|7814321]

  regulation

  • constitutive during vegetative growth [Pubmed|15758244]

  sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]: sigma factor, [Pubmed|18179421], in [regulon|protein:081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|22211522], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• GP891 (''recU''::''cat''), available in [wiki|Jörg Stülke]'s lab [pubmed|22178973]
• BKE22310 ( ''recU''::''ermC''), (available at the [http://bgsc.org/ BGSC] and in [wiki|Fabian Commichau]'s lab) [pubmed|28189581]
• BP784 ( ''recU''::''ermC''), (available in [wiki|Fabian Commichau]'s lab)
• 1A895 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A895&Search=1A895 BGSC]
• BKE22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT,  downstream forward: _UP4_TGATTAACGAAAGGTTGAGA
• BKK22310 (Δ[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22310 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACTTTCTCATCCTCCTTAT,  downstream forward: _UP4_TGATTAACGAAAGGTTGAGA

References

Reviews

• 22933559,9442895,38052445,40874739

Original Publications

• 9642195,16024744,15317759,11810266,19730681,19422832,7814321,18179421,16154091,21600217,18684995,16020779,24770420,24792171,14701911,25939832,27903910,30050509,31876108,23356868,34768753,23356868,36574412,41298506

RecU in other organisms

• 23356868