frlN

168

[metabolite|fructosevaline] [wiki|ABC transporter] (permease)

locus: BSU_32590

Molecular weight: 32.68 kDa

pI: 5.29

Protein length: 292 aa

Gene length: 879 bp

function: uptake of [metabolite|fructosevaline]

product: [metabolite|fructosevaline] [wiki|ABC transporter] (permease)

essential: no

synonyms: frlN, yurN

List of homologs in different organisms, belongs to COG1175 (Galperin et al., 2021)

Gene

Coordinates: 3,348,825  3,349,703

The protein

Protein family

• [wiki|Binding-protein-dependent transport system permease family] (according to UniProt)
• [wiki|MalFG subfamily] (according to UniProt)

[wiki|Domains]

• [wiki|ABC transmembrane type-1 domain] (aa 66-282) (according to UniProt)

Structure

• [PDB|4TQU] (from Sphingomonas sp., 28% identity) [pubmed|26235029]
• [AF|O32155]

[wiki|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD]-[gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|frlP]

  description: [Pubmed|23175651,21347729,22383849]

  regulation

  • induced in the presence of [metabolite|fructosevaline] ([protein|412A1DBBE7F87765DEBEEAAE02A047636A354D5E|frlR]) [pubmed|41165420]
  • repressed during growth in the presence of branched chain amino acids ([protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]) [Pubmed|12618455]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [pubmed|12618455], [pubmed|18083814], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
  • [protein|412A1DBBE7F87765DEBEEAAE02A047636A354D5E|frlR]: repression, [Pubmed|41165420,21398478], in [regulon|protein:412A1DBBE7F87765DEBEEAAE02A047636A354D5E|frlR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [pubmed|22383849], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

  additional information

  • the [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD]-[gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|frlP] operon is strongly downregulated in a [gene|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|cshA]mutant [Pubmed|23175651]
  • the [gene|C87E74FB67B53DD1C68916AA121388C60CE66E92|frlB]-[gene|9E3FF9D0B33D7EFEA3B61FF863BB46CC6DFD4F9B|frlO]-[gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]-[gene|1F245030D0C81DC96FC0642199CA00579CF46D06|frlM]-[gene|C3823AFED3E5C3D1EA04C38D861239468C9E6349|frlD]-[gene|3B9331D6755B8253944AB33521BBF2B3EBA4CAEB|frlP] operon is upregulated in a [gene|F4097349A563503468A2A14F062AEAC532C7917A|rnpM]mutant [Pubmed|23175651]

Biological materials

Mutant

• MGNA-A560 (yurN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/560 NBRP B. subtilis, Japan]
• BKE32590 ([gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE32590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA
• BKK32590 ([gene|8256CAAEC591E40A8CB172FF053E03BFFD5A4B54|frlN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK32590 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAGGGTATCTCATCTCCTT,  downstream forward: _UP4_AAAACCGGAAAGGAGGAGTA

References

• 23175651,21815947,12618455,10092453,21347729,21398478,26235029,36815589,39397791