MccB

• Description: cystathionine lyase/ homocysteine gamma-lyase
  
  

Gene name	mccB
Synonyms	yrhB
Essential	no
Product	cystathionine lyase/ homocysteine gamma-lyase
Function	methionine-to-cysteine conversion
Gene expression levels in SubtiExpress: mccB
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	40 kDa, 5.215
Gene length, protein length	1137 bp, 379 aa
Immediate neighbours	yrhC, mccA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins

This gene is a member of the following regulons

CymR regulon, Spx regulon

The gene

Basic information

• Locus tag: BSU27250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-cystathionine + H₂O = L-cysteine + NH₃ + 2-oxobutanoate (according to Swiss-Prot)

• Protein family: trans-sulfuration enzymes family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O05394

• KEGG entry: [3]

• E.C. number: 4.4.1.1

Expression and regulation

• Operon: yrrT-mtnN-mccA-mccB-yrhC PubMed

• Expression browser: mccB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed in the presence of cysteine (CymR) PubMed

• Regulatory mechanism:
  • CymR: transcription repression PubMed
  • Spx: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:
  • 1A942 ( mccB::kan), PubMed, available at BGSC
  • 1A945 ( mccB::kan), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: