gerT

168

spore coat protein, involved in germination

locus: BSU_19490

Molecular weight: 18.40 kDa

pI: 6.34

Protein length: 157 aa

Gene length: 474 bp

function: germination

product: spore coat protein

essential: no

synonyms: gerT, yozR

List of homologs in different organisms

Gene

Coordinates: 2,124,021  2,124,494

Phenotypes of a mutant

• mutants respond poorly to multiple germinants, impaired in early stage of germination [Pubmed|17720779]

The protein

Structure

• [AF|Q7WY67]

[wiki|Localization]

• spore coat, first assembled in foci, then spread around the developing spore in a [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|cotE]-dependent manner [Pubmed|17720779]

Expression and Regulation

Operons

• genes: [gene|43877BAA7A3CD4F2891B02BD27D7F26BD651B44C|gerT]

  description: [Pubmed|22383849]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK], [protein|search|GerE]) [Pubmed|17720779]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE]: repression, [Pubmed|17720779], in [regulon|protein:19228BAD44E6DA3DE908DC390FDF628C18D94E65|gerE regulon]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK]: sigma factor, [Pubmed|17720779], in [regulon|protein:24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|sigK regulon]

Biological materials

Mutant

• BKE19490 ([gene|43877BAA7A3CD4F2891B02BD27D7F26BD651B44C|gerT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE19490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTTCACATCCTTTC,  downstream forward: _UP4_TAAAAAACGGCCTGCAATCG
• BKK19490 ([gene|43877BAA7A3CD4F2891B02BD27D7F26BD651B44C|gerT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK19490 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTCTTCACATCCTTTC,  downstream forward: _UP4_TAAAAAACGGCCTGCAATCG

References

• 17720779