thiU

168

thiamine [wiki|ECF transporter] (binding protein)

locus: BSU_13240

Molecular weight: 21.88 kDa

pI: 6.05

Protein length: 200 aa

Gene length: 603 bp

function: thiamine uptake

product: thiamine [wiki|ECF transporter] (binding protein)

essential: no

synonyms: thiU, ykoF

List of homologs in different organisms

Gene

Coordinates: 1,391,040  1,391,642

The protein

Structure

• [PDB|1SBR] (complex with thiamine),  [PDB|1S99]
• [AF|O34911]

[wiki|Localization]

• membrane associated (via [protein|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[protein|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]) [Pubmed|16291685]

Expression and Regulation

Operons

• genes: [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]

  description: [Pubmed|16291685]

  regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]

  regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: RNA switch, via [wiki|RNA switch], in [regulon|protein:E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

Biological materials

Mutant

• MGNA-A757 (ykoF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/757 NBRP B. subtilis, Japan]
• BKK13240 ([gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13240 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTCTCCGACCTCCTGTT,  downstream forward: _UP4_AGAAAAAACAGAAAGCAGGG

References

• 16291685,12376536,15451668