sco

168

accessory lipoprotein required for assembly of the Cu(A) center of [protein|72D5442C0EF3B404F7B615366E357B68C863CEB6|cytochrome c oxidase caa3]

locus: BSU_21750

Molecular weight: 21.50 kDa

pI: 4.81

Protein length: 193 aa

Gene length: 582 bp

function: maturation of [protein|72D5442C0EF3B404F7B615366E357B68C863CEB6|cytochrome c oxidase caa3]

product: synthesis of cytochrome oxidase protein

essential: no

synonyms: sco, ypmQ

List of homologs in different organisms, belongs to COG1999 (Galperin et al., 2021)

Gene

Coordinates: 2,291,703  2,292,284

The protein

Catalyzed reaction/ biological activity

• electron transfer to the maturing oxidase [protein|72D5442C0EF3B404F7B615366E357B68C863CEB6|cytochrome c oxidase caa3] [Pubmed|19921776]

Protein family

• SCO1/2 family (single member, according to UniProt)

[wiki|Domains]

• [wiki|Thioredoxin domain] (aa 26-191) (according to UniProt)

[wiki|Cofactors]

• contains copper bound by two cysteines and a histidine residue [Pubmed|19921776]

Structure

• [PDB|1XZO] [pubmed|15723536]
• [AF|P54178]

[wiki|Localization]

• lipoprotein, cell membrane  [Pubmed|19921776]

Expression and Regulation

Operons

• genes: [gene|0337C7B1CFA1C59710A7E6463A370F8C39AAC8EA|sco]-[gene|4722C78DB10289939145BDD079661021160C688C|ypmR]-[gene|6E668E7BCD26FCC9873D538385DAF851D7CEC8FC|ypmS]-[gene|3EDC7B00A30AA32E0F8D4FB40011A4BF08B1AB18|ypmT]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-A882 (ypmQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/882 NBRP B. subtilis, Japan]
• BKE21750 ([gene|0337C7B1CFA1C59710A7E6463A370F8C39AAC8EA|sco]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE21750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGACATCCATCCTTTCCA,  downstream forward: _UP4_TAAGCTAAGAGACTAGGCAT
• BKK21750 ([gene|0337C7B1CFA1C59710A7E6463A370F8C39AAC8EA|sco]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK21750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGACATCCATCCTTTCCA,  downstream forward: _UP4_TAAGCTAAGAGACTAGGCAT

Reviews

• 35630371

References

• 14680962,14766920,16305244,19027886,10837475,19921776,20232870,21333651,21945854,22036877,25192666,21069401,15723536,29131589,12832793,29676768