ileS

168

isoleucyl-tRNA synthetase

locus: BSU_15430

Molecular weight: 104.58 kDa

pI: 5.19

Protein length: 921 aa

Gene length: 2766 bp

function: translation

product: isoleucyl-tRNA synthetase

essential: yes

ec: 6.1.1.5

synonyms: ileS

List of homologs in different organisms, belongs to COG0060 (Galperin et al., 2021)

Gene

Coordinates: 1,613,357  1,616,122

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-isoleucine + tRNA^Ile --> AMP + diphosphate + L-isoleucyl-tRNA^Ile (according to UniProt)

Protein family

• [wiki|Class-I aminoacyl-tRNA synthetase family] (according to UniProt)

Structure

• [PDB|1QU2] (from ''Staphylococcus aureus'', 61% identity) [Pubmed|10446055]
• [AF|Q45477]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation [Pubmed|17981983]

Expression and Regulation

Operons

• genes: [gene|9C29039BB3EE70FEC91C0F0919E579E76EEB193A|ileS]

  description: [pubmed|22383849]

  regulation

  • induced by isoleucine limitation ([wiki|T-box]) [Pubmed|19258532]
  • the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • T-box: RNA switch, [pubmed|19258532], in [regulon|other_regulator:T-box|T-box]

References

• 19258532,10446055,17981983,19233850,28139725,29794222,35357484