kduI

168

5-keto-4-deoxyuronate isomerase

locus: BSU_22130

Molecular weight: 30.98 kDa

pI: 5.11

Protein length: 275 aa

Gene length: 828 bp

function: utilization of galacturonic acid

product: 5-keto-4-deoxyuronate isomerase

essential: no

ec: 5.3.1.17

synonyms: kduI

List of homologs in different organisms, belongs to COG3717 (Galperin et al., 2021)

Gene

Coordinates: 2,325,854 → 2,326,681

The protein

Catalyzed reaction/ biological activity

• 5-dehydro-4-deoxy-D-glucuronate --> 3-deoxy-D-glycero-2,5-hexodiulosonate (according to UniProt)

Protein family

• KduI family (single member, according to UniProt)

Structure

• [PDB|1YWK] (from Enterococcus faecalis, 48% identity)
• [AF|P50843]

Expression and Regulation

Operons

• genes: [gene|CFAEF60AD73F18C8DB5EF042210602B149D51152|kduI]-[gene|FBBDE1E058223D5E8CEB07CAA50940A329C809E4|kduD]

  description: [Pubmed|17322190]

  regulation

  • induced by galacturonate ([protein|search|KdgR]) [Pubmed|17322190]

  regulatory mechanism

  • [protein|891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR]: repression, [Pubmed|17322190], in [regulon|protein:891A32A19D1353BA566FF121BBCA7B986D31D129|kdgR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|17322190], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|17322190], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE22130 (Δ[gene|CFAEF60AD73F18C8DB5EF042210602B149D51152|kduI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE22130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATTTCCCCCTTGGT,  downstream forward: _UP4_ATTCCAATGGATGGGCTGAA
• BKK22130 (Δ[gene|CFAEF60AD73F18C8DB5EF042210602B149D51152|kduI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK22130 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTATATTTCCCCCTTGGT,  downstream forward: _UP4_ATTCCAATGGATGGGCTGAA

References

• 17322190