moeA

168

molybdopterin molybdenumtransferase

locus: BSU_14280

Molecular weight: 46.46 kDa

pI: 5.61

Protein length: 430 aa

Gene length: 1293 bp

function: nitrate respiration

product: molybdopterin molybdenumtransferase

essential: no

ec: 2.10.1.1

synonyms: moeA

List of homologs in different organisms, belongs to COG0303 (Galperin et al., 2021)

Gene

Coordinates: 1,497,192  1,498,484

The protein

Catalyzed reaction/ biological activity

• adenylyl-molybdopterin + H⁺ + molybdate --> AMP + H₂O + Mo-molybdopterin (according to UniProt)

Protein family

• MoeA family (single member, according to UniProt)

Structure

• [PDB|1T3E] (from rat, 35% identity)
• [AF|O31703]

Expression and Regulation

Operons

• genes: [gene|C6DB8499752ED1DBF276C0E46CC561450BED6A40|mobA]-[gene|C163CF34BAA3A37DCC67851DEE5889BB7AD208AB|moeB]-[gene|03DBD906E31D6A8A4D14C9E2DACF0F1AB9F8DAA8|moeA]-[gene|A283C34375EDF495940E2F03C4FB6A9FE841FA1E|mobB]-[gene|12F27FA4DB8232B52271565C4F4937520A4F5C7B|moaE]-[gene|5C3EAEE1287FFB1541A31B31A8B6627E60A4D2EB|moaD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE14280 ([gene|03DBD906E31D6A8A4D14C9E2DACF0F1AB9F8DAA8|moeA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE14280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGCATTCTGACCCCTCCTG,  downstream forward: _UP4_ATCAGAGAAGAGAATGGAAG
• BKK14280 ([gene|03DBD906E31D6A8A4D14C9E2DACF0F1AB9F8DAA8|moeA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK14280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAGCATTCTGACCCCTCCTG,  downstream forward: _UP4_ATCAGAGAAGAGAATGGAAG

References

Reviews

• 23539623

Original Publications

• 22383849