pheT

168

phenylalanyl-tRNA synthetase (beta subunit)

locus: BSU_28630

Molecular weight: 87.76 kDa

pI: 4.86

Protein length: 804 aa

Gene length: 2415 bp

function: translation

product: phenylalanyl-tRNA synthetase (beta subunit)

essential: yes

ec: 6.1.1.20

synonyms: pheT

List of homologs in different organisms, belongs to COG0073 (Galperin et al., 2021)

Gene

Coordinates: 2,927,008 2,929,422

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP + L-phenylalanine + tRNA^Phe --> AMP + diphosphate + H⁺ + L-phenylalanyl-tRNA^Phe (according to UniProt)

Protein family

• phenylalanyl-tRNA synthetase beta subunit family (single member, according to UniProt)

[wiki|Domains]

• [wiki|tRNA-binding domain] (aa 40-155) (accordinig to UniProt)
• B5 domain (aa 409-484) (according to UniProt)
• FDX-ACB domain (aa 710-803) (according to UniProt)

Structure

• [PDB|1PYS] (the [protein|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[protein|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT] complex from ''Thermus thermophilus hb8 mutant'', 37% identity, 56% similarity) [Pubmed|7664121]
• [AF|P17922]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|0ABAEC20F55970C7C1EC5FFE197115E401BF03E8|pheS]-[gene|B255B87EB7A850D1C6B7930A3731DAD6AE0A107D|pheT]

  description: [Pubmed|2127701]

  regulation

  • induced by phenylalanine limitation ([wiki|T-box]) [Pubmed|19258532]
  • the [wiki|T-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

  regulatory mechanism

  • T-box: RNA switch, via tRNA controlled [wiki|RNA switch], in [regulon|other_regulator:T-box|T-box]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|2127701], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

References

• 19258532,2127701,15378759,36815589