ppiB

168

peptidyl-prolyl isomerase

locus: BSU_23360

Molecular weight: 15.11 kDa

pI: 5.47

Protein length: 143 aa

Gene length: 432 bp

function: protein folding

product: peptidyl-prolyl isomerase

essential: no

ec: 5.2.1.8

synonyms: ppiB, cypBS

List of homologs in different organisms, belongs to COG0652 (Galperin et al., 2021)

Gene

Coordinates: 2,435,360  2,435,791

The protein

Catalyzed reaction/ biological activity

• [protein]-peptidylproline (ω=180) --> [protein]-peptidylproline (ω=0) (according to UniProt)

Protein family

• cyclophilin-type PPIase family (single member, according to UniProt)

[wiki|Domains]

• PPIase cyclophilin-type domain (aa 1-143) (according to UniProt)

Structure

• [PDB|2MVZ] (from Geobacillus kaustophilus, 75% identity) [pubmed|25923019]
• [AF|P35137]

Expression and Regulation

Operons

• genes: [gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]

  description: [Pubmed|8022278]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|8022278], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• BKE23360 ([gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGTATGCCTACTTTCT,  downstream forward: _UP4_TAATCGGCAGGCAAAAGGCT
• BKK23360 ([gene|6D38B333DE44EB903E151004BA30F3361CC1BE7C|ppiB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23360 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATTGTATGCCTACTTTCT,  downstream forward: _UP4_TAATCGGCAGGCAAAAGGCT

References

• 9748346,1516692,9297832,8639516,8022278,25923019