proI

168

pyrroline-5-carboxylate reductase

locus: BSU_23800

Molecular weight: 30.24 kDa

pI: 6.7

Protein length: 278 aa

Gene length: 837 bp

function: biosynthesis of proline

product: pyrroline-5-carboxylate reductase

essential: no

ec: 1.5.1.2

synonyms: proI, yqjO

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

Gene

Coordinates: 2,473,151  2,473,987

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺, activity is biologically not relevant [pubmed|28824574]

Protein family

• [wiki|pyrroline-5-carboxylate reductase family] [pubmed|28824574]

[wiki|Cofactors]

• NADPH [pubmed|28824574]

Structure

• [PDB|5BSE] (from ''Medicago truncatula'', 33% identity) [pubmed|26579138]
• [AF|P54552]

Paralogous protein(s)

• [protein|542871C77BE841AA59A65FFE8A44AB85BF9180F0|proH]

Expression and Regulation

Operons

• genes: [gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]

  description: [Pubmed|21784929]

  regulation

  • induced by proline limitation ([protein|search|T-box]) [Pubmed|21233158]

  regulatory mechanism

  • T-box: RNA switch, in [regulon|other_regulator:T-box|T-box]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21233158], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-C395 (yqjO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2393 NBRP B. subtilis, Japan]
• BKE23800 ([gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGATATCCTCCTTCTAT,  downstream forward: _UP4_TAGATGTAAGGACAAAAACA
• BKK23800 ([gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23800 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGATATCCTCCTTCTAT,  downstream forward: _UP4_TAGATGTAAGGACAAAAACA
• GP3748 ([gene|93000B579F630E9F0C6EED40D6661E5E15141FDE|proI]::aphA3 trpC2) available in the [wiki|Stülke] lab

Expression vectors

• pGP3799: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
• pGP3861: expression of Strep-''proI'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab

References

Reviews

• 25367752

Original Publications

• 19258532,21233158,11418582,21784929,28752945,28824574,26579138