yjiC

168

UDP-dependent glycosyltransferase, macrolide glycosyltransferase

locus: BSU_12220

Molecular weight: 43.83 kDa

pI: 4.65

Protein length: 392 aa

Gene length: 1179 bp

function: synthesis of bacillaene

product: UDP-dependent macrolide glycosyltransferase

essential: no

synonyms: yjiC

List of homologs in different organisms, belongs to COG1819 (Galperin et al., 2021)

Gene

Coordinates: 1,292,557  1,293,735

The protein

Protein family

• UDP-glycosyltransferase family (with [protein|BD82663B1BF2763D2D2DE710C2896E4155419324|ydhE] and [protein|6D808BC20ADB6CA789288D50CA816D6BEE22CFC3|yojK], according to UniProt)

Structure

• [PDB|7BOV] [pubmed|33152360]
• [PDB|2IYA] (from Streptomyces antibioticus, 37% identity) [pubmed|17376874]
• [AF|O34539]

Expression and Regulation

Operons

• genes: [gene|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|yjiB]-[gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]

  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A345 (yjiC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/345 NBRP B. subtilis, Japan]
• BKE12220 ([gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE12220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT,  downstream forward: _UP4_TAAAAACATAAAAACCGAAA
• BKK12220 ([gene|02927D2CC9F44B99DB307DDFED2DD52DE2196120|yjiC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK12220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT,  downstream forward: _UP4_TAAAAACATAAAAACCGAAA

References

• 25354260,28315700,17376874,29338263,31726169,31786106,33310191,36657220,33152360,39098192,39120838,40256532