ymaD

168

putative peroxiredoxin

locus: BSU_17280

Molecular weight: 16.13 kDa

pI: 5.2

Protein length: 150 aa

Gene length: 453 bp

function: protection against oxidative stress

product: putative peroxiredoxin

essential: no

synonyms: ymaD

List of homologs in different organisms, belongs to COG1764 (Galperin et al., 2021)

Gene

Coordinates: 1,864,225  1,864,677

Phenotypes of a mutant

• strongly increased the sensitivity of the cells to H₂O₂ [Pubmed|25649915]

The protein

Protein family

• osmC/ohr family (with [protein|C36DD4B670D803C5700428F62E6CA7ED127E4B41|ohrB] and [protein|869E46AECF6249610F8541E403D834284B62171D|ohrA], according to UniProt)

Structure

• [PDB|3I07] (from Vibrio cholerae, 25% identity)
• [AF|O31790]

Expression and Regulation

Operons

• genes: [gene|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD]

  description: [Pubmed|22383849]

• genes: [gene|DF95DED9A6E2160C151D7664C951B198EEA225E8|pghC]-[gene|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD]

  description: [Pubmed|22383849]

Biological materials

Mutant

• GP3166 [gene|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD]::mls trpC2 available in [wiki|Jörg Stülke]'s lab
• MGNA-B069 (ymaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1068 NBRP B. subtilis, Japan]
• BKE17280 ([gene|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGCCTCCTTTTT,  downstream forward: _UP4_GGTGGAGAATAAAGAAAACA
• BKK17280 ([gene|0785B4C70C3DD00EFB8BCDD91EF4F98EF4372186|ymaD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17280 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTGTTTTGCCTCCTTTTT,  downstream forward: _UP4_GGTGGAGAATAAAGAAAACA

References

• 22383849,25649915