View source for PBP43

[[File:pBP43.png|250px|thumb|right|'''pBP43: click to enlarge''']]

'''General comments'''

Integrative [[plasmid]] for the fusion of the monomeric variant of GFP A206K to the C-terminus of a protein, keeping the control of the expression under its natural promoter
* ''E.coli'': multicopy plasmid, select for ampicillin
* ''B. subtilis'': no ori, select for spectinomycin

'''Hints for the usage'''
* amplify the last 600bp of your gene of interest, 
* skip the Stopp-Codon of the gene in the reverse primer
* take care that the integration is in-frame
* do not use the HindIII site for cloning, as GFP is inserted via this restriction site
* Don't use XbaI and KpnI for cloning. They cut the vector twice!

'''The reference'''
<pubmed>  27708634</pubmed>
==  Return to [[Plasmids]] ==