PBP137

pBP137: click to enlarge

pBP137 cloning region: click to enlarge

Vector for the construction of translational gfp fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in Fabian Commichau's lab

• in E. coli: ampicillin resistance

• in B. subtilis: chloramphenicol resistance

• similar vectors: pAC6, pAC7

sequencing primers:

• KG307: 5’-CACCTTCACCCTCTCCACTGACAG -3’
• PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘