alaS
168
alanine-tRNA synthetase
locus
BSU_27410
Molecular weight
97.08 kDa
pI
4.92
function
translation
product
alanine-tRNA synthetase
essential
yes
ec
6.1.1.7
synonyms
alaS
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG0013 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
2,798,174 2,800,810
Phenotypes of a mutant
essential [Pubmed|12682299]
The protein
Catalyzed reaction/ biological activity
ATP + L-alanine + tRNAAla --> AMP + diphosphate + L-alanyl-tRNAAla (according to UniProt)
Protein family
[wiki|Class-II aminoacyl-tRNA synthetase family] (according to UniProt)
Structure
[PDB|1YFS] (enzyme from ''Aquifex aeolicus'') [Pubmed|15657145]
[PDB|3HXU] (enzyme from ''E. coli'' with alanine) [Pubmed|20010690]
[AF|O34526]
Expression and Regulation
Operons
genes
[gene|8C5C7F9A585AE89D53EAF1DB3A9AB525B9E30B4A|alaS]-[gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|reoM]-[gene|508FC7117B4CE7AF119B3830AE9DC113CB058039|yrrK]-[gene|A0AA5289F5E92A4B2E18E4F3439F2D412208826E|yrzB]
description
[pubmed|22383849]
regulation
induced by alanine limitation ([regulon|T-box|]) [Pubmed|19258532]
regulatory mechanism
T-box: RNA switch, [pubmed|], in [regulon|other_regulator:T-box|T-box]
Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
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Time of last update: 2025-05-09 17:09:20
Author of last update: Jstuelk